Sexy aa couple for female m

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Try out PMC Labs and tell us what you think. Learn More. The prevalence of bacterial vaginosis among lesbians is high. We assessed whether unique Lactobacillus strains were shared by female sex partners. Cultures of vaginal and rectal specimens for detection of Lactobacillus organisms were performed for women who reported having had sex with women. Lactobacilli were identified on the basis of DNA homology and were typed and fingerprinted by repetitive element sequence— based polymerase chain reaction rep-PCR.

Detection of L. The flora of the healthy vagina is dominated byH 2 O 2 -producing Lactobacillus species, predominantly Lactobacillus crispatus and Lactobacillus jensenii [ 1 ]. Bacterial vaginosis BV is characterized by overgrowth of commensal anaerobic flora relative to lactobacilli. BV among heterosexual women is associated with a new male sex partner and unprotected intercourse [ 23 ] and with vaginal intercourse immediately after anal intercourse [ 4 ]. The prevalence of BV among lesbians is high relative to that among heterosexual women [ 56 ], and BV is frequently found in both members of lesbian couples [ 5 ].

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Sexual practices involving digital-vaginal or digital-anal contact might transmit vaginal fluid and may promote abnormal vaginal flora [ 7 ]. Criswell et al. We hypothesized that sexual behaviors that could transfer vaginal fluid might also transmit lactobacilli between female sex partners. Rep-PCR uses repetitive sequences throughout the genome for direct amplification of genomic DNA, generating fingerprint patterns unique to bacterial species or strains [ 10 ].

We defined species-specific distribution of genital Lactobacillus isolates recovered from women who reported having had sex with other women and used rep-PCR fingerprinting to assess whether unique Lactobacillus strains were shared by female sex partners. Women who reported having had sex with another woman during the preceding year were eligible and invited to refer female partners for enrollment. Medical histories were obtained by means of a standardized questionnaire. Vaginal specimens were obtained for Gram stain and for culture by swabbing the lateral vaginal wall; swabs for culture were placed in Port-a-Cul anaerobic transport tubes Becton Dickinsontransported to the University of Washington Anaerobe Laboratory, and set up within 24 h.

Rectal specimens were collected for culture by rotating a swab that was inserted 2 cm into the rectum; swabs were placed in Port-a-Cul transport tubes. Quantitative culture of lactobacilli was performed as ly described [ 11 ]. Gram stains were read using the Nugent scale [ 12 ]. Lactobacilli were identified to the genus Sexy aa couple for female m by means of Gram stain findings and colony morphologic characteristics [ 13 ]. After 48 h of anaerobic incubation, plates were exposed to ambient air.

Each strain was passed at least 1 more time to ensure purity and vigor before procedures and restocking. Strain typing of Lactobacillus was performed using rep-PCR fingerprinting as ly described [ 9 ]. Couples were defined as monogamous if they reported having had no other partners during the 3-month period before enrollment. To characterize each couple by use of a composite measure, we averaged the ages of both partners and the of sex partners reported outside the relationship during the 9-month interval before the monogamous period.

To estimate recent sexual practices in the relationship, we used the most recent report and highest of most recent sex acts if the values obtained from each partner did not agree. For each couple, we selected a pair of participants to serve as control subjects for rep-PCR analysis.

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Study procedures were approved by the University of Washington Human Subjects Research Review Committee, and all participants provided written informed consent. Participants were enrolled in a cross-sectional study described ly [ 5 ]. Overall, These participants did not differ from the entire group of participants with respect to factors such as age, race, and sexual history, but they were less likely to have BV. This difference was expected—the women were selected because they had lactobacilli recovered from genital specimens.

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The distribution of Lactobacillus species by anatomic site is depicted in table 1. For women colonized with L. The overall prevalence of rectal colonization did not differ between L. However, relative to L. Relatively few women with L. Distribution of Lactobacillus strains among women for whom cultures of vaginal and rectal specimens were performed. Relative to women without L. Both members from 72 monogamous partnerships were enrolled. Culture was not performed for 1 woman in the remaining couple. Of the 40 couples with both partners colonized by lactobacilli, 31 had strains available for rep-PCR.

Isolates were not available for the remaining 9 couples because they were enrolled before initiation of the protocol for saving lactobacilli isolates.

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When both partners in a couple were colonized by lactobacilli, L. Of these 23 couples, partners in 18 shared the same L. Women in one of these couples shared an L. Unique patterns are seen for each couple, and no similarity was seen for control subjects. Both partners of couple A were vaginally colonized by 2 different L. Two different strains both vaginally and rectally colonized partner 1 of couple B. One strain colonized both the rectum and vagina of partner 1 of couple C and was only isolated from the vagina of partner 2. The same strain was detected from the vagina of both partners of couple D.

Vaginal and rectal colonization by the same strain was found in both partners of couple E. Partners of couple F did not have a strain in common. However, partner 1 was colonized rectally and vaginally by the same strain. Rep-PCR findings were available for 46 other women in the study. Representative gels of repetitive element sequence— based PCR DNA fingerprints of Lactobacillus crispatus isolates from 18 subjects, of whom 12 were from 6 monogamous couples A through F and 6 were from matched control women who were unpartnered at the time of evaluation AA through FF.

The likelihood of sharing identical lactobacilli was not related to the mean age of the couple, the median of lifetime male sex partners, or the practice, frequency, or timing of other types of sexual behaviors, including oral or anal sexual practices; one exception was reported use of shared vaginal sex toys, for which there was a trend toward an association with sharing identical lactobacilli strains OR, 1.

The distribution of genital Lactobacillus species among lesbian and bisexual women in this study was characterized by a low prevalence of L. Vaginal colonization with L. The rectum may provide a receptive environment for L gasseriand sexual behaviors that allow for exchange of rectal flora may facilitate vaginal colonization with this species. Women with L. Nearly three quarters of the female partners we studied shared strains of lactobacilli with unique rep-PCR patterns, and the likelihood of sharing strains was directly related Sexy aa couple for female m having had fewer female sex partners in the prior year, with an association suggested for increasing duration of partnership and for sexual practices that could efficiently transfer vaginal fluid.

Strains of lactobacilli having identical DNA fingerprinting patterns were commonly shared between the vagina and rectum. These data suggest that sexual behavior affects species-specific genital colonization and that women share vaginal Lactobacillus species with their female partners. Data for heterosexual women indicate that the rectum may serve as a reservoir for vaginal lactobacilli that support normal vaginal flora.

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Antonio et al. Although the likelihood of rectal colonization increased with increasing colony counts from vaginal cultures, rectal colonization occurred in the absence of high-density vaginal colonization in one-third of women. Co-colonization of the vagina and rectum by these species was associated with the lowest prevalence of BV. Of note, the prevalence of L. Our study has limitations. Subjects were self-referred and may not be representative of women who have sex with women.

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Most subjects were white, and our sample size may have been too small for subgroup analysis to detect statistical ificance for some associations. Lactobacillus iners may have been underrepresented in our subjects, as we did not include methods specifically developed to cultivate this fastidious organism [ 15 ]. Our findings suggest areas for future study.

First, a potential role of L. Few studies of BV have assessed anal sexual behaviors as an exposure, but a prospective study identified report of anal intercourse immediately following vaginal intercourse as a ificant risk [ 4 ].

Alternatively, vaginal conditions that promote colonization with L. Second, our observation that female sex partners share identical strains of lactobacilli has implications for vaginal application of probiotics to restore vaginal flora. Vaginal colonization with human-derived L. Potential conflicts of interest: none reported.

Sexy aa couple for female m

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